Part:BBa_K277048

3L.3_23.B2.08

3L.3_23.B2.08 is 748 bases long and is cloned into the pGem-T vector.

3L.3_23.B2.08 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B2.08 is a constituent of 3L.3_23.B2 (along with 3L.3_23.B2.01, 3L.3_23.B2.02, 3L.3_23.B2.03, 3L.3_23.B2.04, 3L.3_23.B2.05, 3L.3_23.B2.06, 3L.3_23.B2.07, 3L.3_23.B2.09, 3L.3_23.B2.10, 3L.3_23.B2.11, and 3L.3_23.B2.12.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL045C (124..+2406) Protein of unknown function%3B green fluorescent protein (GFP)-fusion protein localizes to the ER%3B interacts with Gal80p%3B YCL045C is not an essential gene

forward_primer YCL045C_tagf1v1 (346..373)

reverse_primer YCL045C_tagr1v1 (679..706)

Sequence (the first 748 bases correspond to coordinates 34805..35552 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]